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Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode

Received: 16 November 2021     Accepted: 3 December 2021     Published: 23 March 2022
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Abstract

Background. The redox-active protein, myoglobin (Mb), exchanges electrons very slowly with bare electrodes. Just like on a highly oriented pyrolytic graphite (HOPG) bare electrode, electron transfer between myoglobin (Mb) and a bare glassy carbon (GC) electrode was not observed. The myoglobin (Mb) in di-dodecyl dimethyl ammonium bromide (DDAB) film immobilized on glassy carbon electrode surface had a good charge transport, allowing Mb to be used as a redox catalyst for multi-electron transfer reactions. Objective. We report here the myoglobin on a glassy carbon (GC) electrode as a catalyst for the multi-electron reduction of bisulfite in aqueous buffered solutions. Methods. Coulometry and chronoamperometry are used as tools to probe the Mb/DDAB film on GC electrode as an effective electro-catalyst for the multi-electron reduction of bisulfite. Results. Variation in current with time of bisulfite reduction followed first-order reaction kinetics. The heterogeneous electron transfer rate constant of the film and catalytic rate constant for the reduction of bisulfite were determined. Conclusion. The study confirmed that bisulfite was the reactive species and the catalytic reduction reaction at Mb/DDAB film followed the EC’ catalytic mechanism.

Published in American Journal of Chemical Engineering (Volume 10, Issue 2)
DOI 10.11648/j.ajche.20221002.11
Page(s) 18-22
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Mb/DDAB Films, Glassy Carbon (GC) Electrode, Electro-catalysis, EC’ Catalytic Mechanism

References
[1] A. A. Santos, S. S. Venceslau, F. Grein, W. D. Leavitt, C. Dahl, D. T. Johnston, I. A. C. Pereira, Science 2019, 350, 1541–1545.
[2] F. Grein, A. R. Ramos, S. S. Venceslau, I. A. C. Pereira, Biochim. Biophys. Acta 2018, 1827, 145–160.
[3] J. Simon, P. M. H. Kroneck, Adv. Microb. Physiol. 2015, 62, 45–117. Nassar, A F.; Williams, W.; Rusling, J F.; Electron Transfer from Electrodes to Myoglobin: Facilitated in Surfactant Films and Blocked by Adsorbed Biomacromolecules, Anal. Chem. 1995, 67, 14, 2386–2392.
[4] B. R. Crane, L. M. Siegel, E. D. Getzoff, Biochemistry 1997, 36, 12120–12137. Ma, H.; Naifei, H.; Nassar, A F.; Ma. W. C.; Electro active myoglobin films grown layer-by-layer with poly (styrenesulfonate) on pyrolytic graphite electrodes, Langmuir, 2000, 16, 11, 4969–4975.
[5] Rusling, J. F.; Nassar, A. E. F.; Enhanced electron transfer for myoglobin in surfactant films on electrodes, J. Am. Chem. Soc. 1993, 115, 11891-11897.
[6] T. F. Oliveira, C. Vonrhein, P. M. Matias, S. S. Venceslau, I. A. C. Pereira, M. Archer, J. Biol. Chem. 2008, 283, 34141– 34149. Zhong. Z.; Rusling, J. F.; Electron transfer between myoglobin and electrodes in thin films of phosphatidylcholines and dihexadecylphosphate Biophysical Chemistry, 1997, 63, 133-146.
[7] Tanaks, K. T.; Tamamushi, R. T.; Electrochemical study of the polycrystalline gold electrode in dimethylsulphoxide J. Electroanal. Chem., 1987, 236, 305-311.
[8] King, B. C.; Hawakridge, F. M.; Hoffman, A. E; Electrochemical studies of cyanometmyoglobin and metmyoglobin: implications for long-range electron transfer in proteins J. Am. Chem. Soc., 1992, 114, 10603-10608.
[9] Nassar, A. E. F.; Bobbitt, J. M.; Stuart, J. D.; Rusling, J. F.; Catalytic Reduction of Organohalide Pollutants by Myoglobin in a Biomembrane-like Surfactant Film. J. Am. Chem. Soc., 1995, 117, 10986-10993.
[10] Lin, R.; Bayachou, M.; Greaves, J.; Farmer, P. J.; Nitrite Reduction by Myoglobin in Surfactant Films, J. Am. Chem. Soc., 1997, 119, 12689-12690.
[11] Chen, S. M.; Tseng, C. C.; The characterization and bioelectrocatalytic properties of hemoglobin by direct electrochemistry of DDAB film modified electrodes, Electrochim. Acta, 2004, 49, 1903-1914.
[12] Kline, M. A.; Barley, M. H.; Meyer, T. I..; Electrocatalytic reduction of bisulfite to hydrogen sulfide based on a water-soluble iron porphyrin, Iorg. Chem., 1987, 26, 2196-2197.
[13] Sheidt, W. R.; Lee, Y. J.; Finnegan, M. G.; Reactions of sulfur dioxide with iron porphyrinates and the crystal structure of (hydrogen sulfato) (tetraphenyl porphinato) iron(III) hemibenzene solvate, Iorg. Chem., 1988, 27, 4725-4730.
[14] Reynolds, M. S.; Holm, R. H.; Binding of oxysulfur anions to macrocyclic iron(II,III): [(Fe(TPP))2SO4] and Fe(Me6-4, 11-dieneN4)(S2O5)] Iorg. Chim. Acta, 1989, 155, 113-123.
[15] Daum, M. P.; Lenhard, J. R.; Rolison, D. R.; Murray, R. W.; Diffusional charge transport through ultrathin films of radiofrequency plasma polymerized vinylferrocene at low temperature, J. Am. Chem Soc., 1980, 102, 4649-4652. Daum, M. P.; Murray, R. W.; Charge-transfer diffusion rates and activity relationships during oxidation and reduction of plasma-polymerized vinyl-ferrocene films, J. Phys. Chem., 1981, 85, 389-396.
[16] Nicholson, R. S.; Theory and Application of Cyclic Voltammetry for Measurement of Electrode Reaction Kinetics. Anal Chem., 1965, 37, 11, 1351–1355.
[17] Jarza̧bek, G.; Borkowska, Z.; Electrochemical study of the polycrystalline gold electrode in dimethylsulphoxide, Electroanal. Chem., 1987, 295-303.
[18] Peerce, P. J.; Bard, A. J.; Poylmer film electrodes-5, Electroanal. Chem., 1980, 108, 121-136.
[19] Peerce, P. J.; Bard, A. J.; Poylmer film electrodes-5, Electroanal. Chem., 1980, 114, 89-103.
[20] Oyama, N. A.; Anson F. C.; Electrochemical responses of multiply-charged transition metal complexes bound electrostatically to graphite electrode surfaces coated with polyelectrolytes, Electrochem. Soc., 1980, 127, 247-263.
[21] Okamoto, Y.; Suzuki, K.; Ohta, K.; Hatada, H.; Yuki, H.;, Optically active poly(triphenylmethyl methacrylate) with one-handed helical conformation. J. Am. Chem. Soc., 1979, 101, 547-556.
[22] Schroeder, A. H.; Kaufman, F. B.; Engler, E. M.; Kramer, S. R.; Chambers, J. Q.; The influence of polymer morphology on polymer film electrochemistry, J. Am. Chem. Soc., 1980, 102, 483-496.
[23] Daum, M. P.; Murray, R. W.; Increasing the rate of charging of redox polymer films extended surface electrodes, J. Electroanal. Chem., 1979, 103, 289 -303.
[24] Rudolph, A. M.; Theoretical relations among rate constants, barriers, and Broensted slopes of chemical reactions, J. Phys. Chem., 1978, 82, 403-416.
[25] Bard, A. J.; Faulkner, L. R; In Electrochemical Methods: Fundamentals and Applications, Wiley, New York, 2006.
[26] Radalla, A. M.; Rahman, M. H.; Ryan, M. D.; Catalytic Reduction of Bisulfite by Myoglobin/Surfactant Films, Electroanalysis, 2017, 29, 2437-2443.
[27] Bard, A. J Faulkner, L. R; Electrochemical Methods, Wiley, New York, 1980.
[28] Laviron, E.; A. C. polarography and faradaic impedance of strongly adsorbed electroactive species: Part I. Theoretical and experimental study of a quasi-reversible reaction in the case of a Langmuir isotherm J. Electroanal. Chem. 1979, 61. 19-23.
[29] Bard, A. J.; Faulkner, L. R; Electrochemical Methods, Fundamentals and Applications 2nd ed., Wiley, New York, 2001.
[30] Rocklin, R. D.; Murray, R. W.; Kinetics of electrocatalysis of dibromoalkyl reductions using electrodes with covalently immobilized metallotetraphenyl porphyrins, J. Phys. Chem., 1981, 85, 2104-2112.
[31] Andrieux,. P.; Dumas-Bouchiat, J. M.; Savéant, J. M.; Catalysis of electrochemical reactions at redox polymer electrodes: Kinetic model for stationary voltammetric techniques, J. Electroanal. Chem., 1982, 131, 1-36.
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    Abdelatty Mohamed Radalla. (2022). Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode. American Journal of Chemical Engineering, 10(2), 18-22. https://doi.org/10.11648/j.ajche.20221002.11

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    ACS Style

    Abdelatty Mohamed Radalla. Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode. Am. J. Chem. Eng. 2022, 10(2), 18-22. doi: 10.11648/j.ajche.20221002.11

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    AMA Style

    Abdelatty Mohamed Radalla. Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode. Am J Chem Eng. 2022;10(2):18-22. doi: 10.11648/j.ajche.20221002.11

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  • @article{10.11648/j.ajche.20221002.11,
      author = {Abdelatty Mohamed Radalla},
      title = {Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode},
      journal = {American Journal of Chemical Engineering},
      volume = {10},
      number = {2},
      pages = {18-22},
      doi = {10.11648/j.ajche.20221002.11},
      url = {https://doi.org/10.11648/j.ajche.20221002.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20221002.11},
      abstract = {Background. The redox-active protein, myoglobin (Mb), exchanges electrons very slowly with bare electrodes. Just like on a highly oriented pyrolytic graphite (HOPG) bare electrode, electron transfer between myoglobin (Mb) and a bare glassy carbon (GC) electrode was not observed. The myoglobin (Mb) in di-dodecyl dimethyl ammonium bromide (DDAB) film immobilized on glassy carbon electrode surface had a good charge transport, allowing Mb to be used as a redox catalyst for multi-electron transfer reactions. Objective. We report here the myoglobin on a glassy carbon (GC) electrode as a catalyst for the multi-electron reduction of bisulfite in aqueous buffered solutions. Methods. Coulometry and chronoamperometry are used as tools to probe the Mb/DDAB film on GC electrode as an effective electro-catalyst for the multi-electron reduction of bisulfite. Results. Variation in current with time of bisulfite reduction followed first-order reaction kinetics. The heterogeneous electron transfer rate constant of the film and catalytic rate constant for the reduction of bisulfite were determined. Conclusion. The study confirmed that bisulfite was the reactive species and the catalytic reduction reaction at Mb/DDAB film followed the EC’ catalytic mechanism.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode
    AU  - Abdelatty Mohamed Radalla
    Y1  - 2022/03/23
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajche.20221002.11
    DO  - 10.11648/j.ajche.20221002.11
    T2  - American Journal of Chemical Engineering
    JF  - American Journal of Chemical Engineering
    JO  - American Journal of Chemical Engineering
    SP  - 18
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2330-8613
    UR  - https://doi.org/10.11648/j.ajche.20221002.11
    AB  - Background. The redox-active protein, myoglobin (Mb), exchanges electrons very slowly with bare electrodes. Just like on a highly oriented pyrolytic graphite (HOPG) bare electrode, electron transfer between myoglobin (Mb) and a bare glassy carbon (GC) electrode was not observed. The myoglobin (Mb) in di-dodecyl dimethyl ammonium bromide (DDAB) film immobilized on glassy carbon electrode surface had a good charge transport, allowing Mb to be used as a redox catalyst for multi-electron transfer reactions. Objective. We report here the myoglobin on a glassy carbon (GC) electrode as a catalyst for the multi-electron reduction of bisulfite in aqueous buffered solutions. Methods. Coulometry and chronoamperometry are used as tools to probe the Mb/DDAB film on GC electrode as an effective electro-catalyst for the multi-electron reduction of bisulfite. Results. Variation in current with time of bisulfite reduction followed first-order reaction kinetics. The heterogeneous electron transfer rate constant of the film and catalytic rate constant for the reduction of bisulfite were determined. Conclusion. The study confirmed that bisulfite was the reactive species and the catalytic reduction reaction at Mb/DDAB film followed the EC’ catalytic mechanism.
    VL  - 10
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemistry, Faculty of Science, Beni Suef University, Beni Suef, Egypt

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